plpc n flag vector Search Results


plpc n flag vector  (Addgene inc)
93
Addgene inc plpc n flag vector

Plpc N Flag Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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meox1-flag expression vector  (Thermo Fisher)
90
Thermo Fisher meox1-flag expression vector
A. Seven genes were chosen from the PBX1 ChIP target gene list, and occupancy of each promoter by PBX1 was validated by ChIP-qPCR analysis. ChIP was performed using either IgG or anti-PBX1 antibody, and the immunoprecipitated DNA was subjected to qPCR analysis using primers flanking the peak of the PBX1 bound region. B. Transcriptional regulation of these target genes by PBX1 or <t>MEOX1</t> was assessed using siRNA and RT-qPCR analysis. Expression of all seven target genes are significantly down-regulated by PBX1 siRNA as compared to control siRNA (Student's t -test, p <0.01). Except for ZHX2, MEOX1 siRNA significantly downregulated expression of the tested genes (Student's t -test, p <0.01). C. ChIP-qPCR validation of GATA1 and MEOX1 binding near the PBX1-bound sequences. ChIP was performed using IgG, anti-GATA1, or anti-MEOX1 antibody and the immunoprecipitated DNA was subjected to qPCR using same primers as in A . Except for binding of MEOX1 to the ZHX2 promoter, binding of GATA1 or MEOX1 to gene promoters is significantly higher than binding of IgG to the same promoters (Student's t -test, p <0.01).
Meox1 Flag Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mutant serpinb3 with amino acids 340–345 deleted (d6)  (Addgene inc)
90
Addgene inc mutant serpinb3 with amino acids 340–345 deleted (d6)
A. Seven genes were chosen from the PBX1 ChIP target gene list, and occupancy of each promoter by PBX1 was validated by ChIP-qPCR analysis. ChIP was performed using either IgG or anti-PBX1 antibody, and the immunoprecipitated DNA was subjected to qPCR analysis using primers flanking the peak of the PBX1 bound region. B. Transcriptional regulation of these target genes by PBX1 or <t>MEOX1</t> was assessed using siRNA and RT-qPCR analysis. Expression of all seven target genes are significantly down-regulated by PBX1 siRNA as compared to control siRNA (Student's t -test, p <0.01). Except for ZHX2, MEOX1 siRNA significantly downregulated expression of the tested genes (Student's t -test, p <0.01). C. ChIP-qPCR validation of GATA1 and MEOX1 binding near the PBX1-bound sequences. ChIP was performed using IgG, anti-GATA1, or anti-MEOX1 antibody and the immunoprecipitated DNA was subjected to qPCR using same primers as in A . Except for binding of MEOX1 to the ZHX2 promoter, binding of GATA1 or MEOX1 to gene promoters is significantly higher than binding of IgG to the same promoters (Student's t -test, p <0.01).
Mutant Serpinb3 With Amino Acids 340–345 Deleted (D6), supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gateway lr clonase ii enzyme mix  (Thermo Fisher)
90
Thermo Fisher gateway lr clonase ii enzyme mix
A. Seven genes were chosen from the PBX1 ChIP target gene list, and occupancy of each promoter by PBX1 was validated by ChIP-qPCR analysis. ChIP was performed using either IgG or anti-PBX1 antibody, and the immunoprecipitated DNA was subjected to qPCR analysis using primers flanking the peak of the PBX1 bound region. B. Transcriptional regulation of these target genes by PBX1 or <t>MEOX1</t> was assessed using siRNA and RT-qPCR analysis. Expression of all seven target genes are significantly down-regulated by PBX1 siRNA as compared to control siRNA (Student's t -test, p <0.01). Except for ZHX2, MEOX1 siRNA significantly downregulated expression of the tested genes (Student's t -test, p <0.01). C. ChIP-qPCR validation of GATA1 and MEOX1 binding near the PBX1-bound sequences. ChIP was performed using IgG, anti-GATA1, or anti-MEOX1 antibody and the immunoprecipitated DNA was subjected to qPCR using same primers as in A . Except for binding of MEOX1 to the ZHX2 promoter, binding of GATA1 or MEOX1 to gene promoters is significantly higher than binding of IgG to the same promoters (Student's t -test, p <0.01).
Gateway Lr Clonase Ii Enzyme Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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resource source identifier pspax2 addgene  (Addgene inc)
98
Addgene inc resource source identifier pspax2 addgene
A. Seven genes were chosen from the PBX1 ChIP target gene list, and occupancy of each promoter by PBX1 was validated by ChIP-qPCR analysis. ChIP was performed using either IgG or anti-PBX1 antibody, and the immunoprecipitated DNA was subjected to qPCR analysis using primers flanking the peak of the PBX1 bound region. B. Transcriptional regulation of these target genes by PBX1 or <t>MEOX1</t> was assessed using siRNA and RT-qPCR analysis. Expression of all seven target genes are significantly down-regulated by PBX1 siRNA as compared to control siRNA (Student's t -test, p <0.01). Except for ZHX2, MEOX1 siRNA significantly downregulated expression of the tested genes (Student's t -test, p <0.01). C. ChIP-qPCR validation of GATA1 and MEOX1 binding near the PBX1-bound sequences. ChIP was performed using IgG, anti-GATA1, or anti-MEOX1 antibody and the immunoprecipitated DNA was subjected to qPCR using same primers as in A . Except for binding of MEOX1 to the ZHX2 promoter, binding of GATA1 or MEOX1 to gene promoters is significantly higher than binding of IgG to the same promoters (Student's t -test, p <0.01).
Resource Source Identifier Pspax2 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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algorithms lasx software leica n a incucyte sx5 live cell analysis instrument sartorius  (Danaher Inc)
86
Danaher Inc algorithms lasx software leica n a incucyte sx5 live cell analysis instrument sartorius
A. Seven genes were chosen from the PBX1 ChIP target gene list, and occupancy of each promoter by PBX1 was validated by ChIP-qPCR analysis. ChIP was performed using either IgG or anti-PBX1 antibody, and the immunoprecipitated DNA was subjected to qPCR analysis using primers flanking the peak of the PBX1 bound region. B. Transcriptional regulation of these target genes by PBX1 or <t>MEOX1</t> was assessed using siRNA and RT-qPCR analysis. Expression of all seven target genes are significantly down-regulated by PBX1 siRNA as compared to control siRNA (Student's t -test, p <0.01). Except for ZHX2, MEOX1 siRNA significantly downregulated expression of the tested genes (Student's t -test, p <0.01). C. ChIP-qPCR validation of GATA1 and MEOX1 binding near the PBX1-bound sequences. ChIP was performed using IgG, anti-GATA1, or anti-MEOX1 antibody and the immunoprecipitated DNA was subjected to qPCR using same primers as in A . Except for binding of MEOX1 to the ZHX2 promoter, binding of GATA1 or MEOX1 to gene promoters is significantly higher than binding of IgG to the same promoters (Student's t -test, p <0.01).
Algorithms Lasx Software Leica N A Incucyte Sx5 Live Cell Analysis Instrument Sartorius, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Journal: Cell reports

Article Title: SerpinB3 drives cancer stem cell survival in glioblastoma

doi: 10.1016/j.celrep.2022.111348

Figure Lengend Snippet:

Article Snippet: These included a wild-type SerpinB3 in the pLPC-N Flag vector (Addgene, #12521) as well as an empty vector and a mutant SerpinB3 with amino acids 340–345 deleted (Δ6).

Techniques: Recombinant, Plasmid Preparation, Blocking Assay, Polymer, Cell Viability Assay, Enzyme-linked Immunosorbent Assay, Staining, Selection, shRNA, Control, Software

A. Seven genes were chosen from the PBX1 ChIP target gene list, and occupancy of each promoter by PBX1 was validated by ChIP-qPCR analysis. ChIP was performed using either IgG or anti-PBX1 antibody, and the immunoprecipitated DNA was subjected to qPCR analysis using primers flanking the peak of the PBX1 bound region. B. Transcriptional regulation of these target genes by PBX1 or MEOX1 was assessed using siRNA and RT-qPCR analysis. Expression of all seven target genes are significantly down-regulated by PBX1 siRNA as compared to control siRNA (Student's t -test, p <0.01). Except for ZHX2, MEOX1 siRNA significantly downregulated expression of the tested genes (Student's t -test, p <0.01). C. ChIP-qPCR validation of GATA1 and MEOX1 binding near the PBX1-bound sequences. ChIP was performed using IgG, anti-GATA1, or anti-MEOX1 antibody and the immunoprecipitated DNA was subjected to qPCR using same primers as in A . Except for binding of MEOX1 to the ZHX2 promoter, binding of GATA1 or MEOX1 to gene promoters is significantly higher than binding of IgG to the same promoters (Student's t -test, p <0.01).

Journal: PLoS ONE

Article Title: Identification of PBX1 Target Genes in Cancer Cells by Global Mapping of PBX1 Binding Sites

doi: 10.1371/journal.pone.0036054

Figure Lengend Snippet: A. Seven genes were chosen from the PBX1 ChIP target gene list, and occupancy of each promoter by PBX1 was validated by ChIP-qPCR analysis. ChIP was performed using either IgG or anti-PBX1 antibody, and the immunoprecipitated DNA was subjected to qPCR analysis using primers flanking the peak of the PBX1 bound region. B. Transcriptional regulation of these target genes by PBX1 or MEOX1 was assessed using siRNA and RT-qPCR analysis. Expression of all seven target genes are significantly down-regulated by PBX1 siRNA as compared to control siRNA (Student's t -test, p <0.01). Except for ZHX2, MEOX1 siRNA significantly downregulated expression of the tested genes (Student's t -test, p <0.01). C. ChIP-qPCR validation of GATA1 and MEOX1 binding near the PBX1-bound sequences. ChIP was performed using IgG, anti-GATA1, or anti-MEOX1 antibody and the immunoprecipitated DNA was subjected to qPCR using same primers as in A . Except for binding of MEOX1 to the ZHX2 promoter, binding of GATA1 or MEOX1 to gene promoters is significantly higher than binding of IgG to the same promoters (Student's t -test, p <0.01).

Article Snippet: The MEOX1-FLAG expression vector was created by subcloning the MEOX1 cDNA (Ultimate ORF clone ID: IOH40231, Invitrogen) into the pLPC-N-FLAG destination plasmid using the Gateway LR Clonase II enzyme mix following the manufacturer's protocol (Invitrogen).

Techniques: Immunoprecipitation, Quantitative RT-PCR, Expressing, Binding Assay

A. HEK293 cells were transfected with PBX1-V5 and/or MEOX1-FLAG expression vectors. Immunoprecipitation and Western blot were performed using epitope tag-specific antibodies. B. Left panel: OVCAR3 cells were transfected with MEOX1 expression vector or control vector. Western blot was performed to test MEOX1 expression (top). The same cells were transfected with PBX1 siRNA or control siRNA and Western blot was performed to test the down-regulation of PBX1 protein (middle). Detection of GAPDH protein was used as a loading control (bottom). Right panel: Relative cell numbers were measured in OVCAR3 cells transfected with MEOX1 cDNA, PBX1 siRNA, control plasmid (pLPC), and control siRNA (siLuc). Student's t -test was used to determine the significance between the MEOX1 over-expressed group and the control group.

Journal: PLoS ONE

Article Title: Identification of PBX1 Target Genes in Cancer Cells by Global Mapping of PBX1 Binding Sites

doi: 10.1371/journal.pone.0036054

Figure Lengend Snippet: A. HEK293 cells were transfected with PBX1-V5 and/or MEOX1-FLAG expression vectors. Immunoprecipitation and Western blot were performed using epitope tag-specific antibodies. B. Left panel: OVCAR3 cells were transfected with MEOX1 expression vector or control vector. Western blot was performed to test MEOX1 expression (top). The same cells were transfected with PBX1 siRNA or control siRNA and Western blot was performed to test the down-regulation of PBX1 protein (middle). Detection of GAPDH protein was used as a loading control (bottom). Right panel: Relative cell numbers were measured in OVCAR3 cells transfected with MEOX1 cDNA, PBX1 siRNA, control plasmid (pLPC), and control siRNA (siLuc). Student's t -test was used to determine the significance between the MEOX1 over-expressed group and the control group.

Article Snippet: The MEOX1-FLAG expression vector was created by subcloning the MEOX1 cDNA (Ultimate ORF clone ID: IOH40231, Invitrogen) into the pLPC-N-FLAG destination plasmid using the Gateway LR Clonase II enzyme mix following the manufacturer's protocol (Invitrogen).

Techniques: Transfection, Expressing, Immunoprecipitation, Western Blot, Plasmid Preparation